Guinea Pig IgG Fc Antibody

SKU: orb347058

Description

Guinea Pig IgG F(c) antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Guinea pig
• Application Notes:
  Anti-Guinea Pig IgG F(c) antibody has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for Anti-guinea Pig IgG F(c) Antibody.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Guinea Pig IgG F(c) fragment
• Purity: Anti-Guinea Pig IgG F(c) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(ab).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Guinea Pig IgG F(c) Antibody, Goat-a-Guinea Pig F(c), Guinea Pig F(c) in Goat, Goat anti-guinea pig F(c) Secondary Antibody.

The 4-h and 7-d PCA reactions during the Challenges/Boosters of Pollen Instillation. On the 20th day after the 1st immunization, sera was obtained to estimate IgG and IgG antibody reactions using 4-h and 7-d PCAs, respectively. As shown in [Fig 4.], a significant effect was observed in 4-h PCA with a titer of 1:64 and 1:32 dilutions. Seven-day PCA was also elevated with a titer of 1:32 dilutions. The 4-h PCA reaction was not abolished with the heat (56°C, 4h) [Fig 5a], but the PCA reaction was significantly abolished by antibody absorbed with IgG-Fc antibody at concentrations of 40 and 100 µg/ml [Fig 5b]. Fig 4. PCA reaction induced by Anti-Cedar Pollen Antibody in Guinea Pigs. Each value shows mean ± SEM (n = 5). Unfilled circle = 4h, black filled circle = 7d. *, ** Significantly different from 7-d sensitized group at P < 0.01 and P < 0.05, respectively. Fig 5. PCA reaction induced by antibody treatment by heat and absorbing with IgG-Fc in Guinea Pigs. (a) Effect of incubation at 56°C for 4h. Each value shows mean ±SEM (n = 5). Unfilled bar = control, black filled bar = incubation at 56°C for 4h. (b) Effect of antibody absorbed with IgG-Fc. Each value shows mean ±SEM (n = 5). *, ** Significantly different from 7-d sensitized group at P < 0.01 and P < 0.05, respectively.