Guinea pig Eosinophil Chemotactic Factor (ECF) ELISA Kit

• Catalog Number: orb779813
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eosinophil Chemotactic Factor(ECF).
• Reactivity: Guinea pig
• Concentration: 500 pg/mL
• Tested applications: ELISA
• Application notes: standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig ECF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig ECF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig ECF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig ECF in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 3.2 pg/mL
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Alternative names: CCL11 ELISA Kit, SCYA11 ELISA Kit, Eotaxin 1 ELISA
• Note: For research use only