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| Catalog Number | orb1146796 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroid Stimulating Hormone Receptor(TSHR). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroid Stimulating Hormone Receptor(TSHR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroid Stimulating Hormone Receptor(TSHR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroid Stimulating Hormone Receptor(TSHR) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Goat |
| Concentration | 2000 pg/mL |
| Application notes | standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat TSHR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat TSHR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat TSHR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat TSHR in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 31.25-2000 pg/mL |
| Sensitivity | 10.8 pg/mL |
| Sample Types | Tissue homogenates, cell lysates and other biological fluids. |
| Alternative names | LGR3, HTSHR-I, Thyrotropin Receptor, TSH Receptor |
| Note | For research use only |
