Goat Sex Hormone Binding Globulin (SHBG) ELISA Kit

SKU: orb866967

Description

Sandwich Goat SHBG ELISA Kit

Research Area

Metabolism Research, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat SHBG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat SHBG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat SHBG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat SHBG in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Goat
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.271 ng/mL
• Concentration: 40 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ABP ELISA Kit, TEBG ELISA Kit, SBP ELISA Kit, Sex steroid-binding protein ELISA Kit, Testis-specific androgen-binding protein ELISA Kit, Testosterone-estradiol-binding globulin ELISA Kit, Testosterone-estrogen-binding globulin ELISA Kit