Goat Luteinizing Hormone (LH) ELISA Kit

SKU: orb779857

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Luteinizing Hormone(LH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Luteinizing Hormone(LH).

Research Area

Metabolism Research, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 mIU/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat LH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat LH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat LH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat LH in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Goat
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 468.75-30000 pg/mL
• Sensitivity: 152.8 pg/mL
• Concentration: 100 mIU/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ICSH ELISA Kit, Lutropin ELISA Kit, Interstitial Cell Stimulating Hormone ELISA Kit