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Goat IgG F(ab')2 Agarose Antibody

SKU: orb346105

Description

Goat IgG F(ab')2 Agarose Antibody

Research Area

Molecular Biology; Cell Biology

Images & Validation

Tested ApplicationsSDS-PAGE
Application Notes
Goat IgG F(ab')2 Agarose has been tested by SDS-PAGE and is suitable for immunoprecipitation and Immunoaffinity purifications.

Key Properties

SourceGoat
Biological OriginGoat
IsotypeIgG F(ab')2
ConjugationAgarose
PurityThis product is an purified Goat IgG isolated from normal Goat Serum digested by the enzyme pepsin and purified by chromatography coupled to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5.0 mg of anti-Goat IgG.

Storage & Handling

StorageStore vial at 4° C prior to opening. DO NOT FREEZE.
Form/AppearanceSuspension of agarose beads
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Goat IgG F(ab')2 fragment Agarose Conjugate, Goat IgG Fab2 Agarose

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Goat IgG F(ab')2 Agarose Antibody

SDS-PAGE of Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane M: 5 µl Opal Prestained Marker. Lane 1: Reduced Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane 2: Reduced Goat IgG F(c) Fragment (p/n orb2652765). Lane 3: Reduced Goat IgG F(ab) Fragment (p/n orb2652763). Lane 4: Reduced Goat IgM Whole Molecule (p/n orb346110). Load: 1 µg for IgG, F(c) and F(ab); 3 µg for IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Goat IgG F(ab')2 Agarose Antibody (orb346105)

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