Goat anti-GGA3 Antibody

• Catalog Number: orb19126
• Category: Antibodies
• Description: Goat polyclonal antibody to GGA3
• Target: GGA3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Canine, Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EQLSTEVGEVDQ
• RRID: AB_10752418
• MW: 78.3; 74.5
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:16000, WB: 0.3 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 45kDa band in human kidney, lung and placenta lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.3kDa according to NP_619525.1 and 74.5kDa according to NP_054720.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GGA3 antibody, anti golgi associated, gamma a
• Note: For research use only
• Entrez: 23163

Immunohistochemical staining of Human Kidney using GGA3 antibody