GGA3 Antibody

SKU: orb19126

Description

Goat polyclonal antibody to GGA3

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:16000, WB: 0.3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 45kDa band in human kidney, lung and placenta lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.3kDa according to NP_619525.1 and 74.5kDa according to NP_054720.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GGA3
• Protein Sequence: EQLSTEVGEVDQ
• Molecular Weight: 78.3; 74.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GGA3 antibody, anti golgi associated, gamma adaptin ear containing, ARF binding protein 3 antibody, anti HGNC:17079 antibody, anti KIAA0154 antibody, anti ADP-ribosylation factor binding protein 3 antibody, anti Golgi-localized, gamma ear-containing, ARF-binding protein 3 antibody

Immunohistochemical staining of Human Kidney using GGA3 antibody

Quick Database Links

Gene Symbol

GGA3

NCBI Gene ID

23163