Goat anti-Cystatin B / Stefin B Antibody

• Catalog Number: orb18776
• Category: Antibodies
• Description: Goat polyclonal antibody to CSTB
• Target: Cystatin B / Stefin B
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QTNKAKHDELTYF
• RRID: AB_10747936
• MW: 11.1
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave no signal but low background in human brain lysates at up to 1 μg/ml. However this used our routine western blotting protocol which we would not expect to reliably detect proteins as small as the predicted size of approx. 11KDa according to NP_000091. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CSTB antibody, anti cystatin B (stefin B) ant
• Note: For research use only
• Entrez: 1476

Western blot analysis of Human Lungs using CSTB antibody