Arachidonate 5-lipoxygenase Antibody

SKU: orb19321

Description

Goat polyclonal antibody to ALOX5

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 85kDa band observed in Human Bone Marrow and Rat Stomach lysates (calculated MW of 77.7.0kDa according to rat NP_036954). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Arachidonate 5-lipoxygenase
• Protein Sequence: ERNKKKQLPYYYLSPD
• Molecular Weight: 78
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti arachidonate 5-lipoxygenase antibody, anti RP11-67C2.3 antibody, anti ALOX5 antibody, anti ALOX5 antibody, anti 5-LO antibody, anti 5LPG antibody, anti LOG5 antibody, anti arachidonic acid 5-lipoxygenase antibody, anti leukotriene A4 synthase antibody

orb19321 (0.3ug/ml) staining of Rat Stomach lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Arachidonate 5-lipoxygenase

NCBI Gene ID

240