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Goat anti-Androgen Receptor Antibody

Catalog Number: orb18847

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb18847
CategoryAntibodies
DescriptionGoat polyclonal antibody to AR
TargetAndrogen Receptor
ClonalityPolyclonal
Species/HostGoat
ConjugationUnconjugated
ReactivityHuman, Mouse
Predicted ReactivityBovine, Canine, Porcine, Rat
Form/AppearanceLiquid
Buffer/Preservatives0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
PurityPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
ImmunogenPeptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence
Protein SequenceEVQLGLGRVYPRPPSC
RRIDAB_10750893
MWApprox 100kDa band observed in Human Cerebellum lysates (calculated MW of 99.2kDa)
Tested applicationsChIP, ELISA, IF, IHC, WB
Dilution rangeELISA: 1:64000, WB: 0.1-0.5 μg/ml, IHC-P: 2-3 μg/ml
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 95kDa band observed in Human Brain and Human Heart lysates (calculated MW of 99kDa according to NP_000035). Recommended concentration: 0.3-2 μg/ml.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesanti androgen receptor antibody, anti dihydrotesto
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NoteFor research use only
Entrez367
Goat anti-Androgen Receptor Antibody

staining of Human Cerebellum (A) and (0.1 μg/ml) negative control Pancreas (B) lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

Goat anti-Androgen Receptor Antibody

2 μg/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Goat anti-Androgen Receptor Antibody

ChIP of 2 μg with 1 μg DHT-treated HEC50 chromatin using the spin column sonication kit (Protein G) measuring FKBP5 enrichment.

Goat anti-Androgen Receptor Antibody

Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Goat anti-Androgen Receptor Antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Goat anti-Androgen Receptor Antibody

Immunostaining of 25μm thick cryosections of PFA-perfused Mouse Hypothalamus, antigen retrieval with citrate buffer Ph 6 at 80°C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.

Goat anti-Androgen Receptor Antibody

Immunostaining of 25μm thick cryosections of PFA-perfused Mouse Amygdala, antigen retrieval with citrate buffer Ph 6 at 80°C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.

  • Goat anti-RAN (aa 197-210) Antibody [orb125135]

    ELISA,  IF,  WB

    Bovine, Human, Mouse, Rat

    Goat

    Polyclonal

    Unconjugated

    100 μg