Goat anti-Androgen Receptor Antibody

• Catalog Number: orb18847
• Category: Antibodies
• Description: Goat polyclonal antibody to AR
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ChIP, ELISA, IF, IHC, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Reactivity: Human, Mouse
• Immunogen: Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence
• Dilution range: ELISA: 1:64000, WB: 0.1-0.5 μg/ml, IHC-P: 2-3 μg/ml
• Form/Appearance: Liquid
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
• Conjugation: Unconjugated
• MW: Approx 100kDa band observed in Human Cerebellum lysates (calculated MW of 99.2kDa)
• Target: Androgen Receptor
• Entrez: 367
• Protein Sequence: EVQLGLGRVYPRPPSC
• RRID: AB_10750893
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
• Alternative names: anti androgen receptor antibody, anti dihydrotesto
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 95kDa band observed in Human Brain and Human Heart lysates (calculated MW of 99kDa according to NP_000035). Recommended concentration: 0.3-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

staining of Human Cerebellum (A) and (0.1 μg/ml) negative control Pancreas (B) lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

2 μg/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

ChIP of 2 μg with 1 μg DHT-treated HEC50 chromatin using the spin column sonication kit (Protein G) measuring FKBP5 enrichment.

Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Immunostaining of 25μm thick cryosections of PFA-perfused Mouse Hypothalamus, antigen retrieval with citrate buffer Ph 6 at 80°C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.

Immunostaining of 25μm thick cryosections of PFA-perfused Mouse Amygdala, antigen retrieval with citrate buffer Ph 6 at 80°C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.