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PIRH2 Rabbit Polyclonal Antibody

Catalog Number: orb2868

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb2868
CategoryAntibodies
DescriptionPIRH2 Rabbit Polyclonal Antibody
TargetRCHY1
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse
Predicted ReactivityBovine, Canine, Equine, Porcine, Rat, Sheep
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human PIRH2 (31-130/261aa)
UniProt IDQ96PM5
MW30 kDa
Tested applicationsFC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50, IF=1:100-500, Flow-Cyt=2ug/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesAndrogen receptor N terminal interacting protein;
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NoteFor research use only
PIRH2 Rabbit Polyclonal Antibody

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-PIRH2 antibody (orb2868), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

PIRH2 Rabbit Polyclonal Antibody

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PIRH2) polyclonal Antibody, Unconjugated (orb2868) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

PIRH2 Rabbit Polyclonal Antibody

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-PIRH2 (orb2868) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 30kD, Observed band size: 30kD.

PIRH2 Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Stomach tissue lysates, Lane 2: Mouse Spleen tissue lysates, Primary: Anti-PIRH2 (orb2868) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kDa, Observed band size: 30 kDa.

PIRH2 Rabbit Polyclonal Antibody

Tissue/Cell: human glioma tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PIRH2 Polyclonal Antibody, Unconjugated (orb2868) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

PIRH2 Rabbit Polyclonal Antibody

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PIRH2 Polyclonal Antibody, Unconjugated (orb2868) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

  • Anti-Pirh2 Antibody [orb667919]

    WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • RCHY1 Rabbit Polyclonal Antibody [orb330270]

    WB

    Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat, Sheep

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • RCHY1 Rabbit Polyclonal Antibody (HRP) [orb2121392]

    WB

    Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep

    Rabbit

    Polyclonal

    HRP

    100 μl
  • RCHY1 Rabbit Polyclonal Antibody (FITC) [orb2121393]

    WB

    Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep

    Rabbit

    Polyclonal

    FITC

    100 μl
  • RCHY1 Rabbit Polyclonal Antibody (Biotin) [orb2121394]

    WB

    Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep

    Rabbit

    Polyclonal

    Biotin

    100 μl