GLUD1 Antibody

SKU: orb750578

Description

GLUD1 antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:4,000 - 1:16,000, IP: 1:100, WB: 1:1,000 - 1:3,000
• Reactivity: Bovine
• Application Notes:
  This antibody has been tested by western blot. Specific conditions for reactivity should be optimized by the end user. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect a band approximately 56 kDa in size corresponding to glutamate dehydrogenase monomer subunit by western blotting in the appropriate cell or tissue extract. Anti-Glutamate Dehydrogenase Antibody is suitable for use in ELISA.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length Glutamate Dehydrogenase protein isolated from Bovine Liver.
• Target: GLUD1
• Purity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 85 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Glutamate Dehydrogenase Antibody, Glutamate dehydrogenase 1 mitochondrial, GDH 1

Metabolic pathways that may be affected by the inhibition of GDH are indicated. mART, mitochondrial ADP-ribosyl transferase.

Western blot analysis is shown using Biorbyt's anti-bovine glutamate dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kDa. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 µm nitrocellulose. The blot was incubated with a 1:1000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5000 for 45 min at room temperature.

Western Blot using Anti-Glutamate Dehydrogenase (Bovine Liver) (RABBIT) Antibody. Two different molecular weight groups of isoforms were detected for MDH1 at about 36 kDa using Anti-MDH1 (pig heart) orb750614 at 1:1500, Fluorescent Cy-5 labelled secondary at 1:500 and for GDH at about 60 kDa using Anti-GDH (bovine liver) orb750578 at 1:1500, anti-rabbit secondary at 1:500. Light intensities revealed unchanged amount of total soluble MDH1 but increased amount of total soluble GDH in AD when compared to controls. Values of light intensities are given as mean ± S.E.M. *p ≤ 0.05.

Quick Database Links

Gene Symbol

GLUD1

UniProt

P00366

RefSeq:32880221