GLUT1 antibody

SKU: orb2276280

Description

GLUT1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Brain: A strong GLUT1 staining of endothelial cells should be seen. Negative Control: Brain: GLUT1 staining should be absent in all cells/structures except blood vessels. Cellular Localization: Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-401R
• Immunogen: Recombinant fragment of human GLUT1 protein (around aa 203-305) (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Erythrocyte/hepatoma glucose transporter; Glucose transporter type-1; GLUT1; GLUT1DS; GLUTB; GT1; GTG1; Gtg3; HepG2 glucose transporter; PED; RATGTG1; Solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1)

Cerebrum grey matter A particularly strong GLUT1 staining of endothelial cells is seen in the brain.

Placenta amnion and chorion Membranous GLUT1 staining is very intense in amnion and chorion cells. Endothelial cells also show a particularly strong staining.

Skin Squamous cell carcinoma showing a strong GLUT1 immunostaining of tumor cells.

Quick Database Links

UniProt

P11166