Glucoraphanin

SKU: orb1296127

Description

Glucoraphanin is the biologically inactive precursor to the potent antioxidant and chemopreventive compound sulforaphane, found in broccoli. It is widely used in research to study Nrf2 pathway activation, oxidative stress, and cancer chemoprevention in both cellular and animal models.

Research Area

Immunology & Inflammation, Metabolism Research, Signal Transduction

Key Properties

• CAS Number: 21414-41-5
• MW: 437.51
• Purity: 99.64% (May vary between batches)
• Formula: C₁₂H₂₃NO₁₀S₃
• SMILES: CS(=O)CCCC\C(S[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O)=N\OS(O)(=O)=O
• Target: Antioxidant,Endogenous Metabolite,Reactive Oxygen Species,ROS
• Solubility: H2O:74 mg/mL (169.14 mM)

Bioactivity

• In Vivo:
  Glucoraphanin reduces weight gain, enhances energy expenditure, improves insulin sensitivity, and glucose tolerance in HFD-fed mice, but it does not exert these effects in Nrf2-/-. It blocks HFD-induced reduction of Ucp1 protein levels in white adipose depots of wild-type mice but not in Nrf2-/-, alleviates HFD-induced hepatic steatosis and oxidative stress, and suppresses HFD-induced proinflammatory activation of macrophages in the liver and adipose tissue . Additionally, pellets containing 0.1% Glucoraphanin (GF) significantly attenuate decreased social avoidance time and sucrose preference in stressed mice .
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• Animal Research:
  Male C57BL/6JSlc mice are 7 weeks of age. The Nrf2 knockout (Nrf2 / ) mouse strain is used in the assay. After 1 week of acclimation, mice are fed normal chow (NC) (containing 2.2% dextrinized cornstarch, 10% kcal from fat), NC containing 0.3% glucoraphanin (NC-GR) (containing 2.2% extract powder), an HFD (containing 2.2% dextrinized cornstarch, 60% kcal from fat), or an HFD containing 0.3% glucoraphanin (HFD-GR) (containing 2.2% extract powder) for 14 weeks. Both the NC and the HFD containing cornstarch or glucoraphanin are prepared. All mice studied are maintained on a 12-h light/dark cycle at 24-26°C with free access to water and food .
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Antioxidant, Glucoraphanin, inhibit, Inhibitor, Reactive Oxygen Species, ReactiveOxygenSpecies

Compound Identifiers

PubChem CID

6602383