GKI-1 HCl

SKU: orb1745254

Description

GKI-1 HCl is a potent inhibitor of Greatwall kinase (GWL), targeting both hGWLFL and hGWL-KinDom, and exhibits a more pronounced inhibitory effect on ROCK1. This small molecule is a valuable research tool for studying mitosis, cell cycle regulation, and cytoskeletal dynamics in biochemical and cellular assays.

Research Area

Cell Biology, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• MW: 306.19
• Purity: 98.51% (May vary between batches)
• Formula: C₁₅H₁₃Cl₂N₃
• SMILES: ClC1=CC=C(NC2=CC=CC(C3=CNN=C3)=C2)C=C1.Cl
• Target: ROCK
• Solubility: DMSO:45 mg/mL (146.97 mM)

Bioactivity

• Target IC50:
  GWL-KinDom (human):4.9 µM|GWL (human):4.9 µM
• In Vitro:
  GKI-1 (25 and 50 µM, 0-8.5 h) reduces ENSA/ARPP19 phosphorylation levels in Hela cells, resulting in a decrease in mitotic events, mitotic arrest/cell death, and cytokinesis failure.
  Read more...

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ROCK1, Greatwall (GWL), GKI-1 HCl, GKI-1 HCl( 2444764-03-6 Free base), 2444764-03-6, 2444764-03-6 Free base

Compound Identifiers

PubChem CID

169553368