GJ103 sodium salt

SKU: orb1303022

Description

GJ103 sodium salt, an active analog of the read-through compound GJ072, facilitates molecular movement in aqueous solutions by reducing surface tension and viscosity. Its pH-lowering effect can influence biochemical processes, supporting its use in cellular and biochemical research applications related to genetic modulation.

Research Area

Cell Biology, Molecular Biology, Signal Transduction

Key Properties

• CAS Number: 1459687-96-7
• MW: 364.36
• Purity: 99.91% (May vary between batches)
• Formula: C₁₆H₁₃N₄NaO₃S
• SMILES: [Na+].COc1cccc(c1)-n1c(SCC([O-])=O)nnc1-c1ccccn1
• Target: ATM/ATR,Others,DNA/RNA Synthesis
• Solubility: 10% DMSO+40% PEG300+5% Tween-80+45% Saline:2.5 mg/mL (6.86 mM);H2O:Soluble;DMSO:50 mg/mL (137.23 mM)

Bioactivity

• In Vivo:
  GJ103 sodium salt is water soluble, which will enable us to evaluate its in vivo activity by systematic administration.
• In Vitro:
  GJ072 induces ATM kinase on both TGA and TAG stop codons and restored ATMpSer1981 autophosphorylation and SMC1pSer966 transphosphorylation as measured by FACS. GJ072 is active in A-T cells with a homozygous TAA mutation. GJ072 is able to induce detectable full-length ATM protein in treated A-T cells. Early structure-activity relationship studies generates eight active analogs of GJ072. Some GJ072 analogs (e.g., GJ103, GJ106, GJ109, and GJ111) consistently demonstrates their activities in all three PTCs by both FCATMpSer1981 and IRIF assays. GJ071 and GJ072 and some of their analogs (such as GJ103) have similar read-through activity as RTC13 or RTC14, but are more tolerable than RTC13 and RTC14 to A-T cells. GJ103 does not show obvious cytotoxicity in A-T cells at concentration as high as 300 μM.
• Cell Research:
  Cell proliferation assay is used to measure cytotoxicity. Cells are seeded into a flat-bottom 96-well plate, including control wells containing complete growth medium alone as blank absorbance readings. After RTC treatment (GJ103), activated-XTT Solution is added into each well, and the cells are returned to the cell culture incubator for 12-14 hours. The absorbance is measured at 480 nM with relevant 630 nM to assess nonspecific readings.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, GJ 103, GJ103, GJ-103, GJ103 sodium, GJ103 sodium salt, GJ-103 sodium salt, Inhibitor, ATR, ATM

Compound Identifiers

PubChem CID

71748052