Ginsenoside Rk1

SKU: orb1220890

Description

Ginsenoside Rk1 is a component created by processing the ginseng plant at high temperatures. Ginsenoside Rk1 has an anti-inflammatory effect suppresses the activation of Jak2/Stat3 signaling pathway and NF-κB. It also has an anti-tumor effect.

Key Properties

• CAS Number: 494753-69-4
• MW: 767.01
• Purity: >98% (HPLC)
• Formula: C₄₂H₇₀O₁₂
• SMILES: [H][C@@]12[C@H](CC[C@@]1(C)[C@]1(C)CC[C@@]3([H])C(C)(C)[C@H](CC[C@]3(C)[C@@]1([H])C[C@H]2O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O)C(=C)CC\C=C(/C)C
• Target: Others
• Solubility: DMSO: 90 mg/mL (117.34 mM)

Bioactivity

• In Vitro:
  Ginsenoside Rk1 (0-40 μM; 6 hours) inhibits MCP-1 and TNF-α mRNA induced by lipopolysaccharide (LPS), expression of IL-1β is inhibited at 40 μM. Ginsenoside Rk1 (0-40 μM; 24 hours) inhibits phosphorylation of JAK2 and STAT3 (Tyr705 and Ser727) in LPS-induced RAW264.7 cells in a dose-dependent manner. Ginsenoside Rk1 (0-160 μM; 48 hours) results in cell viability significant decrease 75.52 ± 2.51% (40 μM), 52.72 ± 2.54% (80 μM), 17.41 ± 2.94% (120 μM)and 12.63 ± 3.24% (160 μM) compared with control. Ginsenoside Rk1 (0-120 μM; 24 hours) increases G0/G1 phase proportion accompanied with S and G2/M phase proportion decrease in MDA-MB-231 cells. Ginsenoside Rk1 (0-120 μM; 24 hours) promotes the percentage of apoptotic cells in a dose-dependent manner, exhibits to reduction of cell number with nucleus fragmentation, condensation and apoptotic body formation. RT-PCR Cell line: RAW264.7 cells Concentration: 10 μM, 20 μM, 40 μM. Incubation time: 6 hours. Result: Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells. Western blot analysis. Cell line: RAW264.7 cells Concentration: 10 μM, 20 μM, 40 μM. Incubation time: 6 hours. Result: Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells Cell Viability Assay Cell line: MDA-MB-231 cells. Concentration: 0 μM, 40 μM, 80 μM, 120 μM. Incubation time: 48 hours. Result: Inhibited MDA-MB-231 cells proliferation in a dose- and time-dependent manner. Cell Cycle Analysis Cell line: MDA-MB-231 cells. Concentration: 0 μM, 40 μM, 80 μM, 120 μM. Incubation time: 24 hours. Result: Induced G0/G1 phase arrest. Apoptosis Analysis Cell line: MDA-MB-231 cells. Concentration: 0 μM, 40 μM, 80 μM, 120 μM. Incubation time: 24 hours. Result: Induced apoptosis in MDA-MB-231 cells.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

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Others