GFP Mouse Monoclonal Antibody

SKU: orb1973166

Description

GFP Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: IF, IP, WB
• Dilution Range: Western Blot 1:1000 – 1:2000, Immunofluorescence 1:300 – 1:500
• Application Notes:
  Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Target: GFP
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

GFP, monoclonal antibody at 1/1000 dilution. Lysates at 30 µg per lane. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1973166 overnight at 4°C.

Immunofluorescence staining of methanol-fixed Neuro cells transfected with GFP fusion protein showing cytoplasmic staining. Green: GFP, monoclonal antibody (orb1973166): 1-500.

Quick Database Links

Gene Symbol

GFP