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GFP-tag Antibody
SKU: orb323045
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Description
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| Tested Applications | IP, WB |
|---|---|
| Dilution range | WB: 1:2000-1:5000, IP: 1:100-1:200 |
| Reactivity | Plant |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | Recombinant protein corresponding to GFP-tag. |
| Purification | Affinity chromatography |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
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Western blot analysis of over-expressed GFP-tagged protein in 293T cell lysate. (Predicted band size: \; Observed band size: \)
Immunoprecipitation of GFP-tagged protein from HEK293T cells transfected with vector overexpressing GFP tag, using Anti-GFP-tag Antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
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Tong Zhang 1, Kun Wang 2, Shengwei Dou 3, Erlin Gao 4, Patrick J Hussey 5, Zongcheng Lin 4, Pengwei Wang Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae Cell Rep, 43(3), 113913 (2024)
Applications
WB
Reactivity
Plant
Li, Chengyang et al. TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy Nat Commun, 13, 5658 (2022)
GFP-tag Antibody (orb323045)
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