General Transforming Growth Factor Beta 1 (TGF-β1) ELISA Kit

• Catalog Number: orb1806563
• Category: Assays and Kits
• Description: General Transforming Growth Factor Beta 1 (TGF-β1) ELISA Kit
• Target: TGF-b1
• Reactivity: All
• UniProt ID: P01137
• Application notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TransformingGrowth Factor Beta 1(TGF-b1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TransformingGrowth Factor Beta 1(TGF-b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TransformingGrowth Factor Beta 1(TGF-b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TransformingGrowth Factor Beta 1(TGF-b1) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Standard: 2000pg/mL
• Range: 31.25-2000pg/mL
• Sensitivity: 18.75 pg/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: Cartilage-inducing factor,CED,Differentiation inhi
• Note: For research use only