General Sialic Acid (SA) ELISA Kit

SKU: orb1817348

Description

The General Sialic Acid (SA) ELISA Kit is designed to quantify General Sialic Acid (SA) in serum, plasma, and other biological fluids. The kit has a detection range of 78.13-5000 µg/mL and a sensitivity of 25.5 µg/mL.

Research Area

Cancer Biology, Gastroenterology & Hepatology, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SA in the samples is then determined by comparing the OD of the samples to the standard curve.

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Key Properties

• Reactivity: All
• Sample Types: Serum, plasma, other biological fluids
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 78.13-5000 µg/mL
• Sensitivity: 25.5 µg/mL
• Concentration: 5000 µg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SA

Quick Database Links

Gene Symbol

SA