You have no items in your shopping cart.
Cart summary
| Catalog Number | orb1817348 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SA in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | All |
| Concentration | 5000 µg/mL |
| Application notes | standard: 5000 µg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SA in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 78.13-5000 µg/mL |
| Sensitivity | 25.5 µg/mL |
| Sample Types | Serum, plasma, tissue homogenates and other biological fluids. |
| Alternative names | SA |
| Note | For research use only |
