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Salmon Calcitonin (SCT) ELISA Kit

Catalog Number: orb781736

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb781736
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Salmon (SCT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Salmon (SCT).
ReactivityAll
Concentration20000 pg/mL
Tested applicationsELISA
Application notesstandard: 20000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SCT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SCT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SCT in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time2.5h
Range312.5-20000 pg/mL
Sensitivity31 pg/mL
Sample Typesbiological agents
Alternative namesCGRP ELISA Kit, Salcatonin ELISA Kit
NoteFor research use only
Salmon Calcitonin (SCT) ELISA Kit