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Catalog Number | orb781736 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Salmon (SCT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Salmon (SCT). |
Tested applications | ELISA |
Reactivity | All |
Range | 312.5-20000 pg/mL |
Assay Time | 2.5h |
Concentration | 20000 pg/mL |
Sensitivity | 31 pg/mL |
Alternative names | CGRP ELISA Kit, Salcatonin ELISA Kit Read more... |
Note | For research use only |
Application notes | standard: 20000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SCT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SCT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SCT in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | biological agents |
Expiration Date | Please enquire. |