General Methionine-Enkephalin (MEK) ELISA Kit

SKU: orb1146756

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Methionine-Enkephalin(MEK) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Methionine-Enkephalin(MEK). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methionine-Enkephalin(MEK) in the samples is then determined by comparing the OD of the samples to the standard curve.

Images & Validation

• Application Notes:
  standard: 8000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with MEK protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MEK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MEK in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: All
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 125.00-8000 pg/mL
• Sensitivity: 77 pg/mL
• Concentration: 8000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Met-enkephalin, Opioid growth factor, OGF