General Lipoxin A4 (LXA4) ELISA Kit

SKU: orb781688

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Lipoxin A4(LXA4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lipoxin A4(LXA4).

Research Area

Infectious Disease & Virology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 40000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LXA4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LXA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LXA4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: All
• Sample Types: serum, plasma, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 625-40000 pg/mL
• Sensitivity: 172.4 pg/mL
• Concentration: 40000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

LX-A4 ELISA Kit