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| Catalog Number | orb1946412 |
|---|---|
| Category | Assays and Kits |
| Description | General Lipopolysaccharides (LPS) ELISA Kit |
| Target | LPS |
| Reactivity | All |
| Application notes | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LPS. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to LPS. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LPS in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Assay Time | 1.5H |
| Range | 1.57-100ng/mL |
| Sensitivity | 0.94 ng/mL |
| Sample Types | Serum, Plasma, Tissue homogenate and Other biological samples |
| Alternative names | Lipopolysaccharides |
| Note | For research use only |
