General Lipopolysaccharides (LPS) ELISA Kit

SKU: orb1946412

Description

General Lipopolysaccharides (LPS) ELISA Kit

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LPS. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to LPS. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LPS in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: All
• Sample Types: Serum, Plasma, Tissue homogenate and Other biological samples
• Assay Type: Competitive
• Assay Time: 1.5H
• Range: 1.57-100ng/mL
• Sensitivity: 0.68 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Lipopolysaccharides

Quick Database Links

Gene Symbol

LPS