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| Catalog Number | orb1088248 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Citrulline(Cit) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Citrulline(Cit). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Citrulline(Cit) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | All |
| Range | 1.57-100 ng/mL |
| Assay Time | 2.5h |
| Concentration | 100 ng/mL |
| Sensitivity | 0.49 ng/mL |
| Alternative names | Citrulline Read more... |
| Note | For research use only |
| Application notes | standard: 100 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cit protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cit. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cit in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, urine and other biological fluids |
| Expiration Date | Please enquire. |
