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General Carnosine (Car) ELISA Kit

Catalog Number: orb781923

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb781923
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Carnosine(Car) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Carnosine(Car).
Tested applicationsELISA
ReactivityAll
Range14.07-900 ng/mL
Assay Time2.5h
Concentration900 ng/mL
Sensitivity3.65 ng/mL
Alternative namesβ-Alanyl-L-Histidine ELISA Kit
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NoteFor research use only
Application notesstandard: 900 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Car protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Car. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Car in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.
General Carnosine (Car) ELISA Kit