General Biotin ELISA Kit

• Catalog Number: orb2667406
• Category: Assays and Kits
• Description: General Biotin ELISA Kit
• Application notes: standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Biotin protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Biotin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Biotin in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 2.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 4.7 pg/mL
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Alternative names: Biotin
• Note: For research use only