General 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) ELISA Kit

• Catalog Number: orb781881
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-Epi Prostaglandin F2 Alpha(8-epi-PGF2a).
• Tested applications: ELISA
• Reactivity: All
• Range: 31.25-2000 pg/mL
• Assay Time: 2.5h
• Concentration: 2000 pg/mL
• Sensitivity: 9.23 pg/mL
• Alternative names: 8-EpiPGF2-α ELISA Kit, 8epi-PGF2a ELISA Kit
• Note: For research use only
• Application notes: standard: 2000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-epi-PGF2a protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-epi-PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-epi-PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma and other biological fluids
• Expiration Date: Please enquire.