General 5-Methyltetrahydrofolate (5-Me-THF) ELISA Kit

• Catalog Number: orb781896
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Methyltetrahydrofolate(5-Me-THF) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Methyltetrahydrofolate(5-Me-THF).
• Tested applications: ELISA
• Reactivity: All
• Range: 1.41-90 ng/mL
• Assay Time: 2.5h
• Concentration: 90 ng/mL
• Sensitivity: 0.44 ng/mL
• Alternative names: 5-Me-THFA ELISA Kit, 5-Me-H4FA ELISA Kit, 5-Me-THF
• Note: For research use only
• Application notes: standard: 90 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Me-THF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Me-THF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Me-THF in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma and other biological fluids
• Expiration Date: Please enquire.