General 5-Hydroxyindoleacetic Acid (5-HIAA) ELISA Kit

SKU: orb781675

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxyindoleacetic Acid(5-HIAA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxyindoleacetic Acid(5-HIAA).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 400 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HIAA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HIAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HIAA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: All
• Sample Types: serum, plasma, urine, cerebrospinal fluid and other biological fluids
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 6.25-400 ng/mL
• Sensitivity: 1.96 ng/mL
• Concentration: 400 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

5HIAA ELISA Kit