General 4-Hydroxynonenal (4-HNE) ELISA Kit

• Catalog Number: orb1806568
• Category: Assays and Kits
• Description: General 4-Hydroxynonenal (4-HNE) ELISA Kit
• Reactivity: All
• Range: 0.63-40 ng/mL
• Assay Time: 1.5H
• Target: 4-HNE
• Sensitivity: 0.38 ng/mL
• Alternative names: 4-HNE, HNE, 4-hydroxy-2-nonenal, 4-Hydroxynonenal
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-Hydroxynonenal(4-HNE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-Hydroxynonenal(4-HNE). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-Hydroxynonenal(4-HNE) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: Serum, Plasma, tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids
• Expiration Date: Please enquire.