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| Catalog Number | orb1806568 |
|---|---|
| Category | Assays and Kits |
| Description | General 4-Hydroxynonenal (4-HNE) ELISA Kit |
| Target | 4-HNE |
| Reactivity | All |
| Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-Hydroxynonenal(4-HNE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-Hydroxynonenal(4-HNE). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-Hydroxynonenal(4-HNE) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Assay Type | Competitive |
| Assay Time | 1.5H |
| Standard | 40 ng/mL |
| Range | 0.63-40 ng/mL |
| Sensitivity | 0.38 ng/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | 4-HNE, HNE, 4-hydroxy-2-nonenal, 4-Hydroxynonenal |
| Note | For research use only |
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