General 1, 5-Anhydroglucitol (1, 5-AG) ELISA Kit

SKU: orb781677

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,5-Anhydroglucitol(1,5-AG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,5-Anhydroglucitol(1,5-AG).

Research Area

Metabolism Research, Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 150 µg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,5-AG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,5-AG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1,5-AG in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: All
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 2.35-150 µg/mL
• Sensitivity: 0.6 µg/mL
• Concentration: 150 µg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

LycoMark ELISA Kit, 1-Deoxy-D-glucose ELISA Kit, 1-Deoxy-D-glucopyranose ELISA Kit, 1,5-Anhydro-D-glucitol ELISA Kit, 1,5-Anhydrosorbitol ELISA Kit, Aceritol ELISA Kit, Polygalytol ELISA Kit