Ganoderic acid D

SKU: orb1222882

Description

Ganoderic acid D is one of the major components in Ganoderma triterpenes. 2. Ganoderic acid D treatment for 48h inhibits the proliferation of HeLa human cervical carcinoma cells with an IC(5) value of 17.3 +/- .3 microM.

Key Properties

• CAS Number: 108340-60-9
• MW: 514.65
• Purity: >98% (HPLC)
• Formula: C₃₀H₄₂O₇
• SMILES: CC(CC(=O)CC(C)C(=O)O)C1CC(=O)C2(C1(CC(=O)C3=C2C(CC4C3(CCC(=O)C4(C)C)C)O)C)C
• Target: Antioxidant
• Solubility: In Vitro: DMSO : 100 mg/mL (194.31 mM)

Bioactivity

• In Vitro:
  Ganoderic acid D can inhibit the growth of numerous cancer cell lines and it inhibits HeLa human cervical carcinoma cells with an IC50 of 17.3 mM. Ganoderic acid D (1-50 μM; 24-72 hours) reduces the cell survival rate in a dose- and time-dependent manner. Ganoderic acid D (10, 50 μM; 24, 48 hours) induces G2/M phase arrest. Ganoderic acid D (10, 50 μM; 24, 48 hours) induces a morphological change typical of apoptosis in HeLa cells. Ganoderic acid D (10 μM; 48 hours) up-regulates 14-3-3E and PRDX3. Cell Viability Assay Cell line: HeLa human cervical carcinoma cell line (CCL-2). Concentration: 1, 5, 10, 20, 50 μM. Incubation time: 24, 48, 72 hours. Result: Reduced the cell survival rate in a dose- and time-dependent manner and had an IC50 value of 17.3 μM for 48 hours treatment. Cell Cycle Analysis Cell line: HeLa human cervical carcinoma cell line (CCL-2). Concentration: 10, 50 μM. Incubation time: 24, 48 hours. Result: Induced G2/M phase arrest. Displayed a cell cycle profile with an elevated G2/M cell population after 24-h treatment with 10 μM. Apoptosis Analysis Cell line: HeLa human cervical carcinoma cell line (CCL-2). Concentration: 10, 50 μM. Incubation time: 48 hours. Result: Induced a morphological change typical of apoptosis in HeLa cells. Western blot analysis. Cell line: HeLa human cervical carcinoma cell line (CCL-2). Concentration: 10 μM. Incubation time: 48 hours. Result: Up-regulated 14-3-3E and PRDX3.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

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Gene Symbol

Antioxidant