GAD65 Rabbit Polyclonal Antibody (BF647)

SKU: orb1576307

Description

GAD65 Rabbit Polyclonal Antibody (BF647) is a BF647 conjugated antibody targeting GAD2. This antibody is suitable for FC, ICC. It exhibits reactivity with Human, Mouse, Rat samples.

Research Area

Amino Acid Metabolism, Glutamate, Metabolism, Neuroscience

Images & Validation

• Tested Applications: FC, ICC
• Dilution Range: Flow-Cyt=1ug/Test, ICC/IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GAD65 (501-585/585aa)
• Target: GAD2
• Purification: Affinity purified by Protein A
• Conjugation: BF647

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

65 kDa glutamic acid decarboxylase; DCE 2; DCE2; GAD 2; GAD 65; GAD-2; GAD-65; GAD 65, GAD2; Glutamate Decarboxylase 2(pancreatic islets and brain 65kDa); Glutamate Decarboxylase 2; Glutamate Decarboxylase 65; Glutamate decarboxylase 65 kDa isoform; Glutamic Acid Decarboxylase 2; Glutamic Acid Decarboxylase 65.

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-GAD65 antibody, Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

GAD2