GAD2 / GAD65 antibody

SKU: orb2276254

Description

GAD2 / GAD65antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Pancreas: A strong GAD2 staining should be seen in pancreatic islet cells. Negative Control: Colon: GAD2 immunostaining should be absent in all cell types Cellular Localization: Intracellular Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: MSVA-602M
• Immunogen: Recombinant human GAD2 (GAD65) protein fragment (around aa 6-99) (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GAD65

Pancreatic neuroendocrine tumor with strong GAD2 immunostaining of tumor cells.

Strong cytoplasmic GAD2 staining of pancreatic islet cells.

Strong GAD2 staining of nerve fibres in the grey matter of the cerebrum.

Quick Database Links

UniProt

Q05329