GAD2/GAD65 Antibody

SKU: orb18998

Description

Goat polyclonal antibody to GAD65

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.5-1.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  This antibody is expected to recognise both reported variants.ELISA: antibody detection limit dilution 1:16000.WB: Approx 65kDa band observed in Human Brain (Cerebral Cortex, Cerebellum and Hippocampus) lysates (calculated MW of 65.4kDa according to NP_000809.1). Recommended concentration: 0.3-1µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GAD2 / GAD65
• Protein Sequence: TLEDNEERMSRLSK
• Molecular Weight: 65.4; 65.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-65 kDa glutamic acid decarboxylase antibody, anti-DCE 2 antibody, anti-DCE2 antibody, anti-DCE2_HUMAN antibody, anti-GAD 2 antibody, anti-GAD 65 antibody, anti-GAD-2 antibody, anti-GAD-65 antibody, anti-Gad2 antibody, anti-Glutamate decarboxylase 2 (pancreas) antibody, anti-Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) antibody, anti-Glutamate decarboxylase 2 antibody, anti-Glutamate Decarboxylase 65 antibody, anti-Glutamate decarboxylase 65 kDa isoform antibody, anti-Glutamic Acid Decarboxylase 2 antibody, anti-Glutamic Acid Decarboxylase 65 antibody, anti-MGC161605 antibody, anti-MGC161607 antibody

Western blot analysis of Human Brain (Hippocampus) lysate using GAD65 antibody

Immunohistochemical staining of Human colon using GAD65 antibody

Immunohistochemical staining of Human Cerebellum using GAD65 antibody

Quick Database Links

Gene Symbol

GAD2 / GAD65

NCBI Gene ID

2572