G0-C14

SKU: orb1982397

Description

G0-C14 is a cationic lipid-like compound employed in the formulation of nanoparticles for nucleic acid and vaccine delivery. It is widely used in research for in vitro transfection studies and in vivo preclinical models to enhance cellular uptake and immunogenicity.

Research Area

Metabolism Research, Signal Transduction

Key Properties

• CAS Number: 1510653-27-6
• MW: 1790.91
• Purity: 98.00% (May vary between batches)
• Formula: C₁₀₆H₂₁₆N₁₀O₁₀
• SMILES: O=C(NCCNCC(O)CCCCCCCCCCCC)CCN(CCC(=O)NCCN(CC(O)CCCCCCCCCCCC)CC(O)CCCCCCCCCCCC)CCN(CCC(=O)NCCNCC(O)CCCCCCCCCCCC)CCC(=O)NCCN(CC(O)CCCCCCCCCCCC)CC(O)CCCCCCCCCCCC
• Target: Liposome
• Solubility: DMSO:80 mg/mL (44.67 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:3.3 mg/mL (1.84 mM)

Bioactivity

• In Vitro:
  G0-C14 has a highly efficient encapsulation ability for mRNA and pDNA, and the encapsulation efficiency can reach more than 95%. The nanoparticles were prepared as follows: 1. PolyHCPT and DSPE-PEG3K were dissolved in DMF and prepared into a homogeneous solution with a concentration of 5 mg/mL. 2. a mixture of siRNA (0.1 nmol/µL in water) and G0-C14 (5 mg/mL, dissolved in DMF) was prepared and mixed according to different N/P molar ratios. Subsequently, this mixture is combined with PolyHCPT and DSPE-PEG3K solutions. 3. The above mixture was added dropwise to 5 mL of deionized water at 1000 rpm with vigorous stirring. 4. The resulting NPs dispersion was transferred to an ultrafiltration unit. 5. Centrifuge at 2800 rpm for 8 minutes at room temperature to remove organic solvents and free compounds.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Compound Identifiers

PubChem CID

166642488