PFKM Antibody

SKU: orb750418

Description

PFKM antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
• Reactivity: Rabbit
• Application Notes:
  This antibody suitable for use in ELISA, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Fructose-6-Phosphate Kinase [Rabbit Muscle]
• Target: PFKM
• Purity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle]. Cross reactivity against Fructose-6-Phosphate Kinase from other sources may occur but has not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 90 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Fructose-6-Phosphate Kinase Antibody, 6 Phosphofructokinase Muscle Type antibody, GSD7 antibody, MGC8699 antibody, PFKA antibody, PFKL antibody, PFKM antibody, PFKP antibody, PFKX antibody, Phosphofructo 1 Kinase Isozyme A antibody, Phosphofructokinase 1 antibody

Western Blot of Anti-PFK Antibody. Analysis of a yeast zwf1 deletion producing different G6PD isoforms from Arabidopsis thaliana. (B) Western blot of crude extracts from strains producing the indicated AtG6PD isoforms. The upper picture shows the results of the blot analyzed with the Odyssey scanner with anti-PFK shown in red and anti-HA detecting the tagged G6PD enzymes in green. Columns in the lower picture show the quantification of G6PD signals normalized to the amount of PFK detected for each lane in the Western blot, setting ScZwf1 at 100%.

Western Blot of Anti-PFK Antibody. Construction of a yeast zwf1 deletion strain, growth phenotypes and analysis of heterologously produced G6PD enzymes. (C) Western blot analysis of crude extracts from strain HOD269-1C (zwf1::kanMX) carrying the genes indicated on a CEN/ARS vector. The upper picture shows the results of the blot analyzed with the Odyssey scanner with anti-PFK shown in red and anti-HA detecting the tagged G6PD enzymes in green. Columns in the lower picture show the quantification of G6PD signals normalized to the amount of PFK detected for each lane in the Western blot, setting ScZwf1 at 100%.

Quick Database Links

Gene Symbol

PFKM

UniProt

P00511

RefSeq:125128