Folinic Acid Calcium Salt Pentahydrate

SKU: orb1224498

Description

Folinic Acid is a reduced folic acid, which is used in combination with other chemotherapy drugs.(In Vitro):MTX alone induces a concentration-related increase in % micronucleated binucleated cells (MNBN) and % aberrant cells (Abs). There is a decrease in nuclear division index (NDI) with increase in MTX concentration. Similarly, the mitotic index (MI) also decreases in all concentrations of MTX tested. The addition of Folinic acid at 50 μg/ mL significantly reduces % MNBN (40-68%) and % Abs (36-77%). Inhibition is also seen at 5 μg/ mL Folinic acid (12 to 54% for MNBN and 20 to 61% for Abs) .(In Vivo):Folinic acid (7.0 mg/kg; intraperitoneal injection; every second day; for 3 weeks; Balb/c young growing male mice) treatment following methotrexate (MTX) administration appears to reverse this growth inhibition (Chronic administration of MTX induces suppression of skeletal growth in mice).

Key Properties

• CAS Number: 6035-45-6
• MW: 601.58
• Purity: >98% (HPLC)
• Formula: C₂₀H₂₁CaN₇O₇·5H₂O
• SMILES: NC1=NC(=O)C2=C(NCC(CNC3=CC=C(C=C3)C(=O)NC(CCC(O)=O)C(O)=O)N2C=O)N1
• Target: Folate Analogue
• Solubility: Water: 40 mg/mL warmed (66.49 mM)

Bioactivity

• In Vivo:
  Folinic acid (7.0 mg/kg; intraperitoneal injection; every second day; for 3 weeks; Balb/c young growing male mice) treatment following methotrexate (MTX) administration appears to reverse this growth inhibition (Chronic administration of MTX induces suppression of skeletal growth in mice). Animal model: 24 Balb/c young growing male mice aged 3 weeks (11.88 ± 0.25 g). Dosage: 7.0 mg/kg. Administration: Intraperitoneal injection; every second day; for 3 weeks. Result: Following methotrexate (MTX) administration appears to reverse this growth inhibition.
• In Vitro:
  MTX alone induces a concentration-related increase in % micronucleated binucleated cells (MNBN) and % aberrant cells (Abs). There is a decrease in nuclear division index (NDI) with increase in MTX concentration. Similarly, the mitotic index (MI) also decreases in all concentrations of MTX tested. The addition of Folinic acid at 50 μg/ mL significantly reduces % MNBN (40-68%) and % Abs (36-77%). Inhibition is also seen at 5 μg/ mL Folinic acid (12 to 54% for MNBN and 20 to 61% for Abs).

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Folate Analogue