FMK 9a

SKU: orb1707305

Description

FMK-9a is a potent and irreversible inhibitor of ATG4B, demonstrating IC50 values of 80 nM and 73 nM in TR-FRET and cellular luciferase reporter assays, respectively. This compound is a valuable chemical tool for investigating autophagy mechanisms in both in vitro and in vivo cancer research contexts.

Research Area

Cell Biology, Protein Biochemistry

Key Properties

• CAS Number: 1955550-51-2
• MW: 392.42
• Purity: 98.97% (May vary between batches)
• Formula: C₂₃H₂₁FN₂O₃
• SMILES: FCC(=O)CNC(=O)[C@H](Cc1ccccc1)NC(=O)c1cccc2ccccc12
• Target: Autophagy,Cysteine Protease,ATG
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (8.41 mM);DMSO:95 mg/mL (242.09 mM)

Bioactivity

• Target IC50:
  Autophagin-1 (LRA assay):73 nM|Autophagin-1 (FRET assay):80 nM
• In Vivo:
  FMK 9a shows moderate solubility and high liver microsome clearances for human and mice of 13.9 and 70 mL/kg/min.
• In Vitro:
  FMK 9a forms a covalent bond with the more reactive thiol group of Cys74 located at the catalytic site of ATG4B thereby inactivating ATG4B proteolytic activity.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, FMK 9a, inhibit, Autophagy, ATG4B, autophagin-1, FRET assay, autophagin-1, LRA assay, CysteineProtease, Cysteine Protease

Compound Identifiers

PubChem CID

127020966