Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP)

• Catalog Number: orb1471702
• Category: Proteins
• Description: Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP)
• Target: CLDN18.2
• Tag: C-Flag Tag
• Reactivity: Human
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• UniProt ID: P56856
• MW: The human full length CLDN18.2 fusion protein has a MW of 54.2 kDa
• Expression System: HEK293
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Alternative names: CLDN18.2
• Research Area: Epigenetics
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

ELISA plates were pre-coated with 0.5 μg/per well purified human CLDN18.2-EGFP-VLP. Serial diluted Anti-CLDN18.2 monoclonal antibody (orb1173630) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for anti- CLDN18.2 monoclonal antibody binding with CLDN18.2-EGFP-VLP is 877.5 ng/ml.

FACS analysis of CLDN18.2 VLP A. Negative Control 1: Expi293 cells were stained with Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP). B. Negative Control 2: Anti-GPRC5D-CAR-Expi293 cells (an irrelevant CAR) were stained with Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP). C. Positive Control: Anti-CLDN18.2-CAR-Expi293 cells were stained with biotin labeled Protein L, followed by streptavidin-APC antibody. D. Anti-CLDN18.2-CAR-Expi293 cells were stained with Fluorescent Human CLDN18.2 Full Length Protein-VLP (EGFP).