Fluorescein (FITC) Monoclonal Antibody

SKU: orb344393

Description

Fluorescein antibody

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA: 1:5,000 - 1:30,000, FC: 1-2 µg/mL, IHC: 1:250 - 1:1,500, WB: 1:500 - 1:3,000
• Application Notes:
  Anti-Fluorescein Antibody has been tested by ELISA, western blot, and immunofluorescence. This product is suitable for immunomicroscopy, in situ hybridization and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 8B9.C6.D3
• Immunogen: Fluorescein conjugated to Mouse IgG
• Purity: Fluorescein Antibody was prepared from mouse ascites by Protein A/G chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Fluorescein conjugated IgG and Fluorescein conjugated Bovine Serum Albumin.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.51mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse Anti-FITC antibody, mouse anti-fluorescein antibody

Immunofluorescence Microscopy of Mouse Anti-Fluorescein antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Gain: 650. Primary antibody: FITC anti-transferrin receptor antibody. Secondary antibody: CF™488A anti-fluorescein secondary antibody at 1 ug/ml for 45 min at RT. Localization/Staining: plasma membrane and endosome.

Immunofluorescence Microscopy of Mouse Anti-Fluorescein antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: FITC anti-transferrin receptor antibody. Secondary antibody: CF™640R anti-fluorescein secondary antibody at 1 ug/ml (left) and 2 µg (right) for 45 min at RT. Localization/Staining: plasma membrane and endosome.

Western Blot of Mouse Anti-Fluorescein Antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Bovine Albumin (BSA) (p/n orb2652777) [0.01 µg]. Lane 3: Bovine Albumin-Fluorescein (BSA-FITC) [0.01 µg]. Primary Antibody: Ms-anti-Fluorescein 1.0 µg/ml overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Mouse HRP (p/n orb347506) at 1:40000 for 30 mins at RT. Block: 5% BLOTTO in 1X PBS for 1hr at RT. Predicted MW: ~60kDa. Observed MW: ~65kDa. Exposure: 0.5sec.