Fluorescein-5-maleimide

SKU: orb1307188

Description

Fluorescein-5-maleimide is a green-fluorescent probe (Ex/Em ~494/519 nm) designed for selectively labeling cysteine thiol groups in proteins and peptides. It is widely used for in vitro bioconjugation, protein folding studies, and tracking biomolecules in cellular imaging applications.

Key Properties

• CAS Number: 75350-46-8
• MW: 427.36
• Purity: 99.79% (May vary between batches)
• Formula: C₂₄H₁₃NO₇
• SMILES: Oc1ccc2c(Oc3cc(O)ccc3C22OC(=O)c3cc(ccc23)N2C(=O)C=CC2=O)c1
• Target: Others
• Solubility: DMSO:131.25 mg/mL (307.12 mM);H2O:< 0.1 mg/mL (insoluble)

Bioactivity

• Cell Research:
  Instructions I. Solution preparation 1. Stock solution: Prepare 1 mM Fluorescein-5-maleimide stock solution using deionized PBS (d-PBS). 2. Working solution: Dilute the stock solution to an appropriate working concentration, usually 0.1–1 μM, according to experimental needs. II. Operation steps 1. Isolate PBMC from venous blood using Ficoll-Paque density gradient centrifugation and wash with PBS. 2. Label the washed PBMC with anti-human CD3 antibody (10 million cells per 100 μL suspension). 3. Stain with 0.1 μM Fluorescein-5-maleimide and analyze by flow cytometry. Notes: 1. Fluorescein-5-maleimide is light-sensitive and should be stored and handled away from light. 2. Storage conditions: -5°C to -30°C, away from light. 3. Before use, make sure the solution is free of particles and avoid the solution from condensing into clumps due to moisture.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Fluorescein5maleimide, Fluorescein-5-maleimide, Fluorescein 5 maleimide, inhibit, Inhibitor, N-(5-Fluoresceinyl)maleimide

Compound Identifiers

PubChem CID

122038