Flucytosine

SKU: orb1310101

Description

Flucytosine (5-FC) is a synthetic fluorinated pyrimidine analog with potent antifungal properties. It is widely used in research to study antifungal mechanisms and combination therapies, both in vitro against susceptible strains and in vivo within animal models of fungal infection.

Research Area

Infectious Disease & Virology

Key Properties

• CAS Number: 2022-85-7
• MW: 129.09
• Purity: 99.83% (May vary between batches)
• Formula: C₄H₄FN₃O
• SMILES: NC1=C(F)C=NC(=O)N1
• Target: Antifungal,Antibiotic
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (7.75 mM);H2O:5 mg/mL (38.73 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:18.52 mg/mL (143.47 mM)

Bioactivity

• In Vivo:
  Lucytosine (≥1.25 mg/L) inhibits the growth of C. neoformans in Sabouraud glucose broth.
• In Vitro:
  In A/JCr mice infected with Cryptococcus neoformans, the combined treatment of Flucytosine with monoclonal antibody 2H1 proved to be more effective than monotherapy, significantly reducing pulmonary colony formation.
• Cell Research:
  J774.16 murine macrophage-like cells plated on 96-well tissue culture plates are incubated with 500 u/mL murine recombinant gamma-interferon overnight at 37 °C. The medium is then replaced with fresh medium containing gamma-interferon 500 μ/mL, LPS 3 mg/L, C. neoformans cells 1.6 × 104/well and Flucytosine 50 mg/L. Plates are incubated for 24 hours at 37 °C. Cell supernatants are collected and cells lysed by adding 0.1 mL sterile distilled water to each well, incubating at room temperature for 30 minutes, and then aspirating and ejecting the lysate with a pipette several times to complete cell disruption. Wells are rinsed with PBS (0.1 mL) and the cell supernatant, and lysate and rinse from each well are pooled, vortexed, diluted 1:50, vortexed again and spread on Sabouraud's dextrose agar for the cfu determination(Only for Reference)

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA synthesis, 5-Fluorocytosine, Antibiotic, NSC 103805, NSC103805, NSC-103805, inhibit, Fluorocytosine, Flucytosine, Fungal, Inhibitor, Ro 2-9915

Compound Identifiers

PubChem CID

3366