Flubendazole

SKU: orb1225334

Description

Flubendazole is an anthelmintic that is used to treat worm infection in humans. It is available OTC in Europe.

Key Properties

• CAS Number: 31430-15-6
• MW: 313.29
• Purity: >98% (HPLC)
• Formula: C₁₆H₁₂FN₃O₃
• SMILES: O=C(OC)NC1=NC2=CC(C(C3=CC=C(F)C=C3)=O)=CC=C2N1
• Target: Microtubule/Tubulin
• Solubility: DMSO: 1 mg/mL (3.19 mM)

Bioactivity

• In Vivo:
  Animal model: Female BALB/c athymic nude mice (6-8 weeks) with HCT116 cells. Dosage: 10 or 30 mg/kg. Administration: Intraperitoneal injection; every other day; 14 days. Result: Markedly reduced the tumor volume. Significantly reduces the protein level of P-STAT3, promotes autophagy and induces apoptosis In vivo.
• In Vitro:
  Cell Viability Assay Cell line: CRC cells (HCT116, RKO and SW480). Concentration: 0-400 μM. Incubation time: 48 h. Result: Effectively reduces the viability of CRC cells (HCT116, RKO and SW480) in a concentration-dependent manner, with an IC50 of 2-5 μM. Apoptosis Analysis Cell line: CRC cells (HCT116, RKO and SW480). Concentration: 0.3, 0.6, 1.2 μM Incubation time: 48 h. Result: Increased the proportion of apoptotic cells in a dose-dependent manner. Dose-dependently effectively increases caspase-3 activity. Cell Autophagy Assay Cell line: CRC cells (HCT116, RKO and SW480). Concentration: 0.3, 0.6, 1.2 μM Incubation time: 24 h. Result: Induced autophagy initiation by inactivating mTOR and P62, and upregulating LC3-I/II, which are classical marker of autophagy.. Western blot analysis. Cell line: CRC cells (HCT116, RKO and SW480). Concentration: 0.3, 0.6, 1.2 μM Incubation time: 24 h. Result: Strongly reduced the expression of phosphorylated STAT3 (P-STAT3) in a dose and time-dependent manner. No obvious change in total STAT3 expression. Decreased expression of MCL1 and survivin in a dose-dependent manner.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NSC 313680 | R-17889 | R-17899

Quick Database Links

Gene Symbol

Microtubule/Tubulin