FITC Antibody

• Catalog Number: orb750041
• Category: Antibodies
• Description: This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this mAb is also suitable for the conversion of fluorescein-labeled detection into immuno-enzymatic assays.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM395
• Tested applications: FACS, IF, IHC-P, WB
• Isotype: Mouse IgG1, kappa
• Immunogen: FITC conjugated to KLH was used as the immunogen for this FITC antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This FITC antibody is available for research use only.
• Storage: Store the FITC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the FITC antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.