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Mast Cell Tryptase Antibody
Description
Images & Validation
−| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution range | WB: 1:100-1000, IHC-P: 1:50-300 |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Mast Cell Tryptase. Please contact us for the exact immunogen sequence. The peptide is available as orb374786. |
| Target | Mast Cell Tryptase |
| Molecular Weight | 31 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Mast Cell Tryptase Recombinant Rabbit Monoclonal Antibody [orb612206]
IF, IHC-Fr, IHC-P, WB
Rat
Human, Mouse
Rabbit
Recombinant
Unconjugated
50 μl, 100 μlMast Cell Tryptase/TPSAB1 Antibody [orb371750]
ELISA, IHC, WB
Human
Rabbit
Polyclonal
Unconjugated
100 μg

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Immunohistochemical staining of paraffin embedded pig large intestines vessels tissue using Mast cell Chymase antibody (5 ug/ml)

IHC-P image of rat colon tissue using anti-Mast cell Chymase (5 ug/ml)

WB analysis of Hela cells (lane 1), mouse stomach (lane 2), mouse lung (lane 3) using Mast Cell Chymase antibody (1 ug/ml)

Immunohistochemical staining of pig large intestines tissue using anti-Mast cell Chymase (5 ug/ml)

Immunohistochemical staining of rat colon tissue using Mast cell Chymase antibody (5 ug/ml)

Western blot analysis of mouse stomach (lane 1), mouse lung (lane 2), mouse small intestine (lane 3) using Mast Cell Chymase antibody (1 ug/ml)

IHC-P staining of rat prostate tissue using anti-Mast cell Chymase (5 ug/ml)
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Protocol Information
Torrente, Franco et al. Intraepithelial lymphocyte eotaxin-2 expression and perineural mast cell degranulation differentiate allergic/eosinophilic colitis from classic IBD J Pediatr Gastroenterol Nutr, 59, 300-307 (2014)
Mast Cell Tryptase Antibody (orb6398)
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