Fisetin

SKU: orb1304898

Description

Fisetin

Research Area

Cell Biology, Epigenetics & Chromatin, Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 528-48-3
• MW: 286.24
• Purity: 97.22%
• Formula: C₁₅H₁₀O₆
• SMILES: OC1=C(OC=2C(C1=O)=CC=C(O)C2)C3=CC(O)=C(O)C=C3
• Target: Sirtuin,TNF,PPAR
• Solubility: Ethanol:2.9 mg/mL (10.13 mM);DMSO:28.6 mg/mL (99.92 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2.87 mg/mL (10.03 mM)

Bioactivity

• In Vivo:
  METHODS: To detect anti-tumor activity in vivo, Fisetin (300 mg/kg) was administered intraperitoneally every two days for 20 days to BALB/c nude mice bearing PANC-1 xenografts. RESULTS: Tumor size was significantly reduced in Fisetin-treated mice. proliferation-associated protein proliferating cell nuclear antigen (PCNA) was significantly reduced in the Fisetin-treated group. fisetin treatment reduced the expression of proteins associated with cell growth and proliferation, including PCNA, Ki67, and phosphorylated histone H3.
• In Vitro:
  METHODS: Human pancreatic cancer cells PANC-1 were treated with Fisetin (25-400 µM) for 24-48 h. Cell viability was measured by CCK-8 assay. RESULTS: Low concentrations of Fisetin (25-50 µM) did not significantly inhibit the viability of PANC-1 cells, and high concentrations inhibited it. METHODS: HeLa cells were treated with Fisetin (20-50 µM) for 24-48 h. Cell cycle was detected by Flow cytometry. RESULTS: Cells treated with 20/30/50 µM Fisetin resulted in a high accumulation of G2/M phase cells. The proportion of G2/M-arrested cells increased from 10.1% to 16.2%, 18.9% and 25.1%, respectively, at 24 h, whereas the proportion of G2/M-blocked cells increased to 30.9%, 36.2% and 56.2%, respectively, at 48 h. Meanwhile, at 48 h, 50 µM of Fisetin put a large proportion of cells in Go/G1 phase.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Fisetin, Inhibitor, TNF Receptor, SIRT, Sirtuin, TNFR, Peroxisome proliferator-activated receptors, PPAR, Tumor Necrosis Factor Receptor

Compound Identifiers

PubChem CID

5281614