FIDAS-5

SKU: orb1309672

Description

FIDAS-5

Research Area

Epigenetics & Chromatin

Key Properties

• CAS Number: 1391934-98-7
• MW: 261.72
• Purity: 97.66%
• Formula: C₁₅H₁₃ClFN
• SMILES: CNc1ccc(\C=C\c2c(F)cccc2Cl)cc1
• Target: Methionine Adenosyltransferase (MAT),Others
• Solubility: DMSO:125 mg/mL (477.61 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (15.28 mM)

Bioactivity

• Target IC50:
  Mat2A:2.1 μM
• In Vivo:
  METHODS: Tumors were induced in athymic nude mice by subcutaneous injection of HT29 CRC cells. FIDAS-5 treatment (20 mg/kg) was administered orally via gavage. The oral efficacy of FIDAS-5 on HT29 tumor xenografts in nude mice was tested by measuring tumors twice weekly using digital calipers. RESULTS FIDAS-5 significantly inhibited xenograft tumor growth with minimal body weight differences.
• In Vitro:
  METHODS: FIDAS-5 (3 μM; 36 hours) was used to treat LS174T cells, and the levels of S-adenosylmethionine (SAM) and S-adenosylhomocysteine ​​(SAH) were observed. RESULTS FIDAS-5 reduced S-adenosylmethionine (SAM) and S-adenosylhomocysteine ​​(SAH) levels in LS174T cells. METHODS: FIDAS-5 (3 μM; 7 days) was used to treat LS174T cells, and the proliferation of LS174T cells was observed. RESULTS FIDAS-5 significantly inhibited the proliferation of LS174T cells. METHODS: FIDAS-5 (3 μM) treatment inhibited LS174T colorectal cancer cells, and the expression of c-Myc and cyclinD1 in LS174T colorectal cancer cells was observed by westernBlot. RESULTS FIDAS-5 inhibited the expression of c-Myc and cyclinD1 in LS174T colorectal cancer cells . METHODS: OPM2 cells were transduced with siMAT2A or scrambled siRNA for 3 days. MAT2A-silenced and parental OPM2 cells were treated with different doses of FIDAS-5 (0.5, 1, 2 μM) to determine whether FIDAS-5 indeed impairs MM cell survival by inhibiting the enzymatic activity of MAT2A. RESULTS The antitumor effect of FIDAS-5 on MAT2A-silenced cells was impaired compared with cells transduced with scrambled siRNA. FIDAS-5 indeed inhibited MM cell survival in part by targeting MAT2A.

Storage & Handling

• Storage: Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-proliferation, anti-cancer, CyclinD1, colorectal, c-Myc, FIDAS 5, FIDAS5, FIDAS-5, Inhibitor, MAT2A, inhibit, Methionine S-adenosyltransferase 2A(MAT2A), SAM, SAH, S-adenosylmethionine, p21WAF1/CIP1

Compound Identifiers

PubChem CID

57521314